INTRACELLULAR LOCALIZATION OF ACID PHOSPHATASE
نویسندگان
چکیده
منابع مشابه
Intracellular Localization of Acid Phosphatase
The acid phosphatase activity of rat liver homogenates and of nuclear and cytoplasmic fractions isolated therefrom (by differential centrifugation) was determined biochemically in a series of experiments. For each liver, Gomori's histochemical test for acid phosphatase was run in parallel. No correlation was found between the biochemical and the histochemical results. According to the former, t...
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Eosinophil (and heterophil) leukocytes of glycogen-induced rabbit peritoneal exudates were fixed for 1(1/2) min in 2% glutaraldehyde and examined for acid phosphatase activity both biochemically and cytochemically. Biochemical assays showed that enzymatic activity had been inhibited by only approximately 10% under these conditions. The cytochemical reaction in the eosinophil was confined to the...
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The alveoli of the mammalian lung are lined by a film of surface-active material (surfactant) which is commonly believed to be a phospholipid (1, 5, 8, 11) . A considerable body of evidence has accumulated which suggests that surfactant is synthesized by the granular pneumonocytes (type II cells) of the alveolar epithelium (1, 9, 10) . Although a number of acid hydrolases have been demonstrated...
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Nonspecific acid phosphatases are a group of enzymes whose activity increases the availability of exogenous and endogenous orthophosphate either through extra- or intracellular hydrolysis of phosphate compounds. Our study demonstrates the activity of acid phosphatases in the filamentous freshwater alga Stigeoclonium tenue. These enzymes were detected following a cerium-based method in which cer...
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Localization of acid phosphatase (ACPase) in rat liver was investigated by immunocytochemical techniques. Rat liver was fixed by perfusion and cut into thick tissue slices, which were embedded in Epon or Lowicryl K4M. For light microscopy (LM), semithin Epon sections were stained for the enzyme ACPase by an indirect immunoenzyme technique. For electron microscopy (EM), ultra-thin Lowicryl K4M s...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1951
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.94.6.535